pf127 solution  (Thermo Fisher)

Journal: Molecular Neurodegeneration

Article Title: Automated longitudinal monitoring of in vivo protein aggregation in neurodegenerative disease C. elegans models

doi: 10.1186/s13024-016-0083-6

Figure Lengend Snippet: a Experimental temperature at the chip center during a worm culture-to-imaging transition, as managed by the active temperature control system, indicating the periods of PF127 injection and chip temperature changes. b Temperature rise in the 15–19 min period in more detail. The axis on the right shows the variation of PF127 solution viscosity (25 % w/v in water) during the transition from 15 to 25 °C in the device. The PF127 sol–gel transition occurs abruptly at about 17 °C, in a time window of ~1 min. Values of PF127 viscosity at the different temperatures are measured through a cone-plate viscometer

Article Snippet: We experimentally determine the viscosity at different temperatures by dispensing a ~2 mL PF127 solution over the bottom plate of a cone-plate viscometer (Bohlin Gemini Malvern, UK) and measuring using a shear rate of 10 s −1 .

Techniques: Imaging, Injection

Journal: Molecular Neurodegeneration

Article Title: Automated longitudinal monitoring of in vivo protein aggregation in neurodegenerative disease C. elegans models

doi: 10.1186/s13024-016-0083-6

Figure Lengend Snippet: a Time-lapse fluorescent pictures of four AM725 transgenic worms, immobilized in a PF127 gel matrix within the culture chambers. Scale bars = 100 μm. b Growth rate of SOD1-YFP aggregates in the body wall muscle cells of each worm, as estimated by measuring YFP expression area across each worm’s body during their immobilization in the gel matrix. c Average protein aggregate/worm area over time. For each worm and each time-point, the aggregate area is normalized by the worm area to take into account the size variability of each worm. A clear time-dependent increase of these values is observed over the period from 43 to 91 h upon loading on chip (day 1 to day 3 of worm adulthood). d Brightfield and fluorescent images of an immobilized worm (worm 1), as taken through a 63× NA 1.4 oil immersion objective 91 h upon worm loading into the device. These pictures allow mapping the aggregate morphology at high spatio-temporal resolution. e Superimposed brightfield and fluorescent images of an immobilized worm (worm 4), as taken through a 63× NA 1.4 oil immersion objective 43 and 60 h upon worm loading into the device. Arrows point at specific SOD1-YFP aggregates, which can be re-identified in subsequent images and tracked over time. Scale bars = 20 μm

Article Snippet: We experimentally determine the viscosity at different temperatures by dispensing a ~2 mL PF127 solution over the bottom plate of a cone-plate viscometer (Bohlin Gemini Malvern, UK) and measuring using a shear rate of 10 s −1 .

Techniques: Transgenic Assay, Expressing